Question: What happens if the promoter and operator trp operon are reversed?

What happens if the promoter and operator in the lac operon are reversed? The operon will always be ON. This will eliminate repression of the operon since binding of repressor to this operator will not interfere with the ability of the RNA polymerase to start transcription of the genes.

What would happen if there was a mutation in the operator of the trp operon?

5. What would happen if the operator sequence of the trp operon contained a mutation that prevented the repressor protein from binding to the operator? In the absence of tryptophan, the genes trpA-E will not be transcribed. In the absence of tryptophan, only genes trpE and trpD will be transcribed.

What happens if there is a nonsense mutation of the regulator gene in the trp operon?

If a lot of trp is present the operon will be repressed. The effect of the nonsense mutations would be revealed under intermediate trp levels where the attenuator normally modulates the transcription termination efficiency.

What would happen if the trp repressor were mutated such that it could no longer bind to the operator?

If the repressor can no longer bind tryptophan, then it cannot bind the operator, and transcription of the trp operon will always be on, whether tryptophan levels in the cell are high or low. The operon is regulated transcriptionally not translationally.

What would happen in the cell if a mutation occurred at the operator preventing binding of the repressor protein?

If a mutation prevented the repressor protein from binding at the operator, then the operon would never be turned off and transcription would occur all the time. This will result in significantly lower levels of transcription of the lac structural genes.

What would be the result of a mutation in the trp operon repressor protein that prevented it from binding tryptophan?

A mutation in the 5ʹ UTR of the trp operon reduces the ability for region 2 to pair with region 3. What would be the effect of this mutation on the trp operon when tryptophan levels are high? The mutation would have no effect on the trp operon.

What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator?

What would happen if the operator sequence of the lac operon contained a mutation that prevented the repressor protein from binding the operator? In the absence of lactose, the lac operon will be transcribed. The cAMP-CAP complex will not increase RNA synthesis. The RNA polymerase will not bind the promoter.

What would happen if there were a mutation in the operator?

If there were a mutation in the operator region of the trp operon, there would be a nucleotide change in the sequence of the operator.

What would happen to the expression of the tryptophan operon if you replaced the key tryptophan codons in the attenuator region with tyrosine codons?

If the tryptophan codons though were replaced with tyrosine codons then the basics of the system would remain the same. However, the operon would be regulated by the concentrations of tyrosine NOT tryptophan. When there are low levels of tyrosine then the operon will be transcribed and translated and vise versa.

What would happen to the regulation of the tryptophan operon in cells that express a mutant form of the tryptophan repressor that?

Transcription of the tryptophan operon would no longer be regulated by the absence or presence of tryptophan; the enzymes would be permanently turned on in 1 & 2 and permanently shut off in 3.

What would happen if there was a mutation in the lac repressor?

a) Most mutations in the operator, the binding site for repressor, lead to lower affinity for the repressor and hence less binding. Thus these mutations allow continued transcription (and thus expression) of the lac operon even in the absence of inducer; this is referred to constitutive expression.

What happens when tryptophan is absent?

When tryptophan is absent, the repressor protein does not bind to the operator and the genes are transcribed. When tryptophan is present in the cell, two tryptophan molecules bind to the trp repressor, which changes shape to bind to the trp operator.

What would happen if bacteria did not have trp R?

What would happen if bacteria did not have trp R? The cell would not be able to break down tryptophan. The cell will gradually produce more tryptophan over time. The cell would not be able to make tryptophan.

What would happen if the operator was removed from the lac operon?

According to the lac operon model proposed by Jacob and Monod, what is predicted to occur if the operator is removed from the operon? The lac operon would be transcribed continuously. Only lacZ would be transcribed. Galactosidase permease would be produced, but would be incapable of transporting lactose.

What would be the outcome of a mutation that prevented DNA binding proteins from being produced?

What would be the outcome of a mutation that prevented DNA binding proteins from being produced? Transcription levels would decrease. Several human diseases are caused by an error in mRNA splicing.

What would happen if the position of the operator region and promoter region were switched in the lac operon?

What happens if the promoter and operator in the lac operon are reversed? The operon will always be ON. This will eliminate repression of the operon since binding of repressor to this operator will not interfere with the ability of the RNA polymerase to start transcription of the genes.

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